Laboratory 3 - Biology


  The Biology laboratory is a facility for the evaluation of biomaterials, surfaces modified through various techniques and compounds synthesized in INFLPR and partner laboratories;

  - Providing testing and characterization services (Microscopy (immunohistochemistry, immunofluorescence)  viability tests, cell proliferation, ELISA, flow cytometry, sorting of cell populations, etc.) to economic agents or institutions that do not have these testing capabilities.

  - Obtaining and testing intelligent surfaces and interfaces for tissue engineering and implantology;

  - Development of study protocols at cellular / intracellular level of various compounds ( including pharmaceuticals ), which can be transferred to users (drug testing laboratories, research centers - CIB ).

L3P1

 

 

 

 

 

 

Convex and concave micro-structured silicone controls the shape, but not the polarization state of human macrophages, V. Malheiro et al, Biomaterials Science, 2016

 

 

 


 The activity of the laboratory falls within the directions 3 and 4 of the themes of the INFLPR strategy;

Dir. 3 -  Nanostructures and advanced materials obtained / characterized by laser, plasma and radiation methods for eco-nano-technologies.
Dir. 4 - Laser, plasma and radiation methods and devices with applications in pharmacology and biomedicine.

Interconnectivity with existing laboratories L3

 

 

 

 

 

 

 

 

 

 

 


Institutional equipment / facilities;


L3P3

  • Microscopy (immunohistochemistry - IHC, immunofluorescence)

  • Feasibility tests, cell proliferation (MTT, MTS, SBR - Sulforhodamine B colorimetric assay; CCK-8 (WST-8) Assay)

  • Research and diagnostic methods (eg ELISA tests - enzyme-linked immunosorbent assay)

  • Flow cytometry and sorting of cell populations (with applications in medical biology: hematology, immunology, oncology, virology, parasitology, bacteriology, endocrinology; pharmacology and toxicology: the effect of immunotherapeutic, chemotherapeutic, antibiotic agents);  

          - Cell pigments: chlorophyll, phycoerythrin; pH, as well as intracellular Mg and ion exchange

          - Enzymatic activity, measurement of cell membrane, mitochondrial potential

          - Cell viability and apoptosis

          - Cell adhesion (pathogen-host cell adhesion)

          - Multiple drug resistance in cancer cells

 

 

 

 

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro, L.E. Sima et al., 2020  

 

 

 

 


   Purchased equipment;


  • Vertical autoclave

          responsible staff - Simona Nistorescu, Valentina Dinca

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

  • Cell biology hood

          responsible staff - Simona Nistorescu, Valentina Dinca

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

  • Freeze dryer

          responsible staff - Simona Nistorescu, Valentina Dinca

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

  • CO2 Incubator

          responsible staff - Simona Nistorescu, Valentina Dinca

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

  • High speed centrifuge with 3 rotors and accessories

          responsible staff - Simona Nistorescu, Valentina Dinca

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

  • Flow cytometer with built-in microscope - Luminex AMNI008

 

L3Eq6

 

 

 

          responsible staff - Simona Nistorescu, Cristina Staicu

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

 

  • Automatic line for cell culture processing and product verification

          responsible staff - Simona Nistorescu, Cristina Staicu

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

  • Multi-mode plate reader with automatic microscope - Clariostar

    L3Eq8

     

 

 

 

 

          responsible staff - Simona Nistorescu, Valentina Dinca

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 

 

  • Fluorescence microscope

         responsible staff - Simona Nistorescu, Valentina Dinca

          users - Simona Nistorescu, Cristina Staicu, Valentina Dinca, external users (based on scheduling)

 


In purchase equipment;


  • Vertical deep freezer with accessories
  • Centrifuge rotor cells and accessories
  • Filtration system
  • Microcentrifuge
  • Cell sonicator-disruptor

 


Laboratory Team 


 

   Acting Head of LaboratoryVDinca

      

   Dr. Valentina Dinca - CSI

   valentina.dinca@inflpr.ro

 

CStaicu

 

 

 

   Drd. Cristina Staicu - ACS

   elena.staicu@inflpr.ro

 

SNistorescu

 

 

   Drd Simona Nistorescu - ACS

   simona.nistorescu@inflpr.ro

 


Perspectives - Research and development activity:


 

  • Development of bio-platforms aimed at cell screening to understand the behavior of cells related to surface chemistry, physical factors (hardness, topography, etc.);

  • Development of test processes and recipes for biomaterials depending on their type;

  • Obtaining and in vitro testing of systems and structures for cellular studies in three-dimensional biomimetic configurations; development of study platforms and identification of compounds that lead to impaired behavior of spheroid systems;

  • Development of bio-interface modification / control strategies;

  • Development of surface modification processes and recipes for in vitro cellular response control;

  • Development of diagnostic platforms, biochips / bio-interfaces for testing allergenic, toxic compounds.